Journal: Antioxidants

Article Title: Bavachin and Corylifol A Improve Muscle Atrophy by Enhancing Mitochondria Quality Control in Type 2 Diabetic Mice

doi: 10.3390/antiox12010137

Figure Lengend Snippet: Bavachin and corylifol A induce the conversion of fast muscle fibers to slow muscle fibers in db/db mice. Twelve week old male db/db mice were administered bavachin (10 mg/kg) or corylifol A (10 mg/kg) for 40 d. ( A ) Representative images of MyHC immunofluorescence in GA muscle. Images were triple labeled for MyHC 1 (blue, type 1 fibers), MyHC 2A (green, type 2A fibers), and MyHC 2B (red, type 2B fibers). Scale bar indicates 50 µm. Images were captured at 600× magnification. ( B ) Proportion of MyHC isoform-specific fibers. Protein expression levels in the GA muscles were determined via western blotting. ( C ) Protein expression levels of MyHC 1, MyHC 2B, troponin I fast skeletal muscle (TnI-FS) and troponin I slow skeletal muscle (TnI-SS). All data are represented as the mean ± S.E.M., n = 6/group; * p < 0.05, ** p < 0.01, *** p < 0.001; nonparametric analysis of variance, followed by Kruskal-Wallis test.

Article Snippet: Subsequently, the fixed tissues were permeabilized in PBS containing 0.2% Triton X-100 at 25 °C for 40 min and incubated at 25 °C with a protein blocking solution (Dako, Santa Clara, CA, USA) for 1 h. For characterization of the skeletal muscle fiber, the sections were incubated overnight at 4 °C in a mixture of BA-F8, SC-71, and BF-F3 antibodies at a ratio of 1:100 (Developmental Studies Hybridoma Bank [DSHB], Houston, TX, USA): anti-MyHC I (BA-F8, mouse IgG2b), anti-MyHC 2A (SC-71, mouse IgG1), and anti-MyHC 2B (BF-F3, mouse IgM).

Techniques: Immunofluorescence, Labeling, Expressing, Western Blot